Partner 1. ENEA (ENEA)
Applied Physics Division
Via Enrico Fermi 45 - 00044 Frascati - Italy

Scientific team

Name	Position		Task
G.P. Gallerano	Responsible for FEL research activity		Project coordination
G. Campurra	Med. Resp. for Health and Safety Service		Blood cell tests - Safety issues
A. Doria	Scientific staff		Optical design and spectroscopy
E. Giovenale	Scientific staff		Near field microscopy, project web-site
G. Messina	Responsible for accelerator activity		Accelerator physics and irradiation set-up
G. Ronci	Technical staff		Instrumentation and controls
M. Bortoli	Technical staff		FEL operation and maintenance
E. Campana	Technical staff		Mechanical design

Objectives
The project will be coordinated by Dr. G.P. Gallerano, who is responsible for the Free Electron Laser (FEL) activity at the Applied Physics Division of the ENEA-Frascati Research Center. The Administrative Staff of the Applied Physics Division will assist the coordinator with consolidated expertise in the management of European contracts.
With its expertise on lasers, optical design and spectroscopy in a wide spectral range extending from the near infrared to the mm-wave region, as well as expertise in ionizing radiation physics, ENEA will devote a Compact FEL operating in the mm wavelength region to the project. The Compact FEL has a tuneability range between 70 and 200 GHz, with peak power of about 10 kW, average power of about 50 mW, line-width 10-4< Dn/n < 10-2 depending of the operating mode, and pulse duration of 4 ms. A new FEL source with extended tuning range (70 GHz to 1 THz) is currently being completed in the framework of a national programme funded by the Italian Ministry of Scientific Research. At ENEA-Frascati fully equipped facilities for basic biological investigations and safe handling of biological materials are also available. The ENEA team will contribute to all workpackages of the project, by performing pump-probe time-resolved spectroscopic measurements on enzyme reactions in collaboration with UFRANK, by setting up the exposure systems for the irradiation of blood cells and liposomes in the biological assays performed by ICEmB.

Workplan
The ENEA team will mainly contribute to the project workpackages as described below:

Workpackage 1 (17 person-months)
WP-1 is mainly devoted to spectroscopic investigations of biological samples in the range 100 GHz to 20 THz. ENEA will make its Compact FEL available for measurements in the range from 70 to 200 GHz. After the basic spectroscopic studies will be completed, ENEA will set up a femtosecond Ti:Sapphire laser synchronized to the FEL source, to perform time-resolved spectroscopic measurements on enzyme reactions in collaboration with UFRANK.

Workpackage 2.1 (19 person-months)
ENEA will set up the exposure system for the irradiation of blood cells in collaboration with ICEmB. ENEA will assist ICEmB in the taking the blood samples at Frascati, preparing the cuvettes and exposing them to THz radiation under controlled conditions. ENEA will also provide assistance in the transfer of the samples to IRECE-Naples, where MN and Comet assays will be performed.

Workpackage 2.2 (19 person-months)
ENEA will set up the exposure system for the irradiation of liposomes prepared by ICEmB-IMS. ENEA will collaborate to the kinetic measurements on the CA enzyme reaction